| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Washington, Madison L (10)
|
MRCAS |
32.05 |
32.01 |
-0.04 |
| 2 |
Graham, Kamaria B (10)
|
NJDESC |
41.77 |
35.30 |
-6.47 |
| 3 |
Martinez, Claudia (10)
|
MRASAP |
38.74 |
36.39 |
-2.35 |
| 4 |
Gatterdam, Lyla (10)
|
MRSS |
35.20 |
36.48 |
1.28 |
| 5 |
Mischka, Camilla E (10)
|
MRSS |
35.97 |
38.73 |
2.76 |
| 6 |
Echavarria, Victoria P (10)
|
MRCAS |
36.18 |
38.84 |
2.66 |
| 7 |
Lane, Jordan (10)
|
MRCAS |
34.49 |
39.10 |
4.61 |
| 8 |
Hibbert, Laura (9)
|
MRSS |
40.66 |
39.57 |
-1.09 |
| 9 |
Bahl, Sangeeta (10)
|
MRFAST |
38.29 |
39.83 |
1.54 |
| 10 |
Parasnis, Gabriella A (10)
|
MRSS |
41.09 |
41.80 |
0.71 |
| 11 |
Blitstein, Tali E (9)
|
MRASAP |
47.65 |
42.33 |
-5.32 |
| 12 |
Filieri, Ava M (8)
|
NJUN |
48.11 |
43.85 |
-4.26 |
| 13 |
Ore, Allison C (10)
|
NJUN |
45.07 |
46.70 |
1.63 |
| 14 |
Polanco, Crysol (10)
|
MRFAST |
|
48.86 |
- |
| 15 |
Ratzan, Maya N (10)
|
NJDESC |
53.49 |
51.21 |
-2.28 |
| 16 |
Seidenberg, Olivia F (9)
|
MRSS |
52.14 |
52.21 |
0.07 |